Pond Life: Protozoa

Part I

Amoeba

Watson Bactil Binocular Microscope
Ocular: x8 Compensating
Objective: x40 with Wilska spot
Wilska Phase contrast
Camera: Canon Powershot S50

Image enhanced by use of Paint Shop Pro Artistic effects: Topography

Mayorella

Zeiss GFL Microscope
Objective: Zeiss x40 plan achromat
Ocular: Watson x8 compensating
Circular Oblique Lighting (COL)
Canon Powershot S50
F4.9, ISO 50, Flash

Testate amoeba: Pamphagus

This amoeba has a case or test and filose pseudopodia

Top image:

 

  • Olympus FH microscope
  • Objective: Gillete & Siebert x40 PH
  • Ocular: Watson x8 compensating
  • Phase contrast

 

Lower image:

  • Zeiss GFL Microscope
  • Objective: Zeiss x40 plan achromat
  • Ocular: Watson x8 compensating
  • Circular Oblique Lighting (COL)

 

Arcella

Zeiss GFL Microscope
Objective: Leica EF L X 32
Ocular: Watson X8 Compensating
Brightfield illumination

Sample from jar kept in window for many weeks

Camera: Canon Powershot S50
Flash
F4.9, ISO 200

Nebela

The lower image shows the fine pseudopia that attache the amoeba to its test (shell)

Zeiss GFL Microscope
Objective: Zeiss x25 plan achromat
Ocular: Watson x8 compensating
Circular oblique Lighting (COL)
Sample from garden pond
Camera: Canon Powershot S50
ISO200, F4.9. 1/200 sec

Heliozoa

Heterophrys

Olympus FH Microscope
Objective: Gillette & Siebert x40 PH
Ocular: Watson x8 compensating
Phase contrast
ISO200, F4.9, 1/30 sec
White balance set to tungsten light

Clathrulina

an amoeboid organism with a stalked, perforated test

Stalk not visible in this picture

Zeiss GFL microscope
Objective: Zeiss x25 plan achromat
Ocular: Watson x8 compensating
Brightfield

Camera: Canon Powershot S50, ISO200, F4.9, 1/400 sec

Click here to view an animated .gif of the effects of focus change, demonstating the spherical nature of this organism

 

Euglenid Flagellates: Anisonema


Olympus FH Microscope
Objective: Watson x30 para
Wilska phase contrast stripe
Ocular: Wild x 10 photo
Sample originally collected 28-Dec-2003, Warnham, dipping pond 2

Camera: Aiptek Pocketcam 3Mega

 

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